7. (a) (i) What is an immobilised enzyme?
(ii) Give one advantage of using immobilised enzymes - Leaving Cert Biology - Question 7 - 2020
Question 7
7. (a) (i) What is an immobilised enzyme?
(ii) Give one advantage of using immobilised enzymes.
(b) Answer the following questions in relation to an investigation th... show full transcript
Worked Solution & Example Answer:7. (a) (i) What is an immobilised enzyme?
(ii) Give one advantage of using immobilised enzymes - Leaving Cert Biology - Question 7 - 2020
Step 1
What is an immobilised enzyme?
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Answer
An immobilised enzyme is an enzyme that is fixed (or attached) to a solid support or an inert substance. This technique allows for the enzyme to be reused multiple times while maintaining its activity.
Step 2
Give one advantage of using immobilised enzymes.
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One advantage of using immobilised enzymes is that they can be recovered and reused, leading to cost-effectiveness in industrial applications.
Step 3
Name the enzyme or cell that you used.
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Answer
The enzyme used in this investigation was Sodium alginate or yeast.
Step 4
Describe how you immobilised that enzyme or cell.
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To immobilise the enzyme, I first mixed it with Sodium alginate. Then, I dropped the mixture into Calcium chloride solution, allowing the enzyme to form beads. After that, I left the beads to harden and rinsed them to remove excess calcium.
Step 5
Outline how you used the immobilised enzyme or cell to examine its application.
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The immobilised enzyme beads were added to a reaction vessel. I then tested their activity by adding a matching substrate, observing the resultant product to check for the expected outcome. The results indicated the enzyme's activity level, with controls set to compare results.
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