In one of your laboratory activities you isolated DNA from a plant tissue - Leaving Cert Biology - Question 7 - 2010
Question 7
In one of your laboratory activities you isolated DNA from a plant tissue.
(a) (i) Where in plant cells is DNA found?
(ii) What is meant by DNA profiling?
(b) (i) ... show full transcript
Worked Solution & Example Answer:In one of your laboratory activities you isolated DNA from a plant tissue - Leaving Cert Biology - Question 7 - 2010
Step 1
Where in plant cells is DNA found?
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DNA in plant cells is found in the nucleus, chloroplasts, or mitochondria.
Step 2
What is meant by DNA profiling?
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DNA profiling refers to analyzing or examining an individual's DNA to identify unique patterns that can be compared to others, commonly used for forensic analysis or paternity testing.
Step 3
Give one reason why you first chopped the plant material into very small pieces.
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Chopping the plant material increases the surface area or helps to burst the cells, making it easier to extract the DNA.
Step 4
Explain why the detergent was used.
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The detergent was used to break down cell membranes, allowing access to the DNA by disrupting the lipid bilayer.
Step 5
How was this mixture heated?
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The mixture was heated in a water bath.
Step 6
Why was this mixture heated?
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Heating the mixture helps to inactivate enzymes that could degrade the DNA during the extraction process.
Step 7
What would happen to the DNA if the mixture was blended for longer than 3 seconds?
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If the mixture was blended for longer than 3 seconds, the DNA would be sheared or fragmented, making it difficult to isolate intact DNA strands.
Step 8
Why was protease added?
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Protease was added to digest the proteins associated with the DNA, helping to unravel it for easier extraction.
Step 9
After filtration, where was the DNA of your plant tissue to be found?
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After filtration, the DNA of the plant tissue would be found in the filtrate or liquid in the test tube.
Step 10
What should you do next to make the DNA visible?
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To make the DNA visible, you should add freezer-cold ethanol or alcohol to the filtrate.
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